Chronic Effects: Prolonged or repeated exposure may affect the liver, kidneys, cardiovascular system, and nervous system. May cause damage to eyesight. May cause mutagenic effects, adverse reproductive effects, and dermatitis. Toxicological Data: Water: Not applicable. Ethanol: LD50 Oral, Rat: 7060 mg/kg LC50 Inhalation, Rat: 124.7 mg/L 4 h Methanol: LD50 Oral, Rat: 5628 mg/kg LD50 Dermal, Rabbit: 15,800 mg/kg LC50 Inhalation, Rat: 87.5 mg/L 6 h Isopropanol: LD50 Oral, Rat: 5045 mg/kg LD50 Dermal, Rabbit: 12,800 mg/kg LC50 Inhalation, Rat: 72.6 mg/L 4 h Methylene Blue: No information found. Potassium Hydroxide: LD50 Dermal, Rabbit: 273 mg/kg Corrosive to eyes and skin based on human and animal data. Symptoms of Exposure: Irritation, nausea, vomiting, diarrhea, visual disturbances, abdominal pain, nervous system effects, drowsiness, dizziness, blindness, metabolic acidosis, unconsciousness. Carcinogenic Effects: No component of this product is considered to be a carcinogen by IARC, ACGIH, NTP, or OSHA. ACGIH: Isopropanol: A4 – Not classifiable as a human carcinogen IARC: Isopropanol: 3 – Not classifiable as to its carcinogenicity to humans 12. ECOLOGICAL INFORMATION Ecotoxicological Data: Water: Not applicable. Ethanol: EC50, Water Flea (Daphnia magna): 7.7 mg/L 48 h LC50, Fathead Minnow (Pimephales promelas): > 100 mg/L 96 h Methanol: EC50, Water Flea (Daphnia magna): > 10,000 mg/L 48 h LC50, Fathead Minnow (Pimephales promelas): > 100 mg/L 96 h Isopropanol: LC50, Western Mosquitofish (Gambusia affinis): >1400 mg/L 96 h Methylene Blue: No information found. Potassium Hydroxide: LC50, Western Mosquitofish (Gambusia affinis): 80 mg/L 96 h Persistence and Degradability: May not be readily biodegradable. Product: Loeffler’s Methylene Blue Revision Date: 10/06/2016 6/8
