Methanol: LD50 Oral, Rat: 5628 mg/kg LC50 Inhalation, Rat: 87.5 mg/L 6 h LD50 Dermal, Rabbit: 15,800 mg/kg Causes reproductive effects based on animal data. Isopropanol: LD50 Oral, Rat: 5045 mg/kg LC50 Inhalation, Rat: 72.6 mg/L 4 h LD50 Dermal, Rabbit: 12,800 mg/kg Crystal Violet: LD50 Oral, Mouse: 96 mg/kg May cause cancer based on animal data. Ammonium Oxalate, Monohydrate: No information found. Symptoms of Exposure: Irritation, unconsciousness, visual disturbances, metabolic acidosis, drowsiness, dizziness, suffocation, shortness of breath, nervous system effects, cardiovascular effects, cough, nausea, vomiting, diarrhea, abdominal pain, constipation, blindness, and respiration effects. Carcinogenic Effects: No component of this product is considered to be a carcinogen by IARC, ACGIH, NTP, or OSHA. ACGIH: Isopropanol: A4 – Not classifiable as a human carcinogen IARC: Isopropanol: 3 – Not classifiable to humans 12. ECOLOGICAL INFORMATION Ecotoxicological Data: Water: Not applicable. Ethanol: EC50, Water Flea (Daphnia magna): 7.7 mg/L 48 h LC50, Fathead Minnow (Pimephales promelas): > 100 mg/L 96 h Methanol: EC50, Water Flea (Daphnia magna): > 10,000 mg/L 48 h LC50, Fathead Minnow (Pimephales promelas): > 100 mg/L 96 h Isopropanol: LC50, Western Mosquitofish (Gambusia affinis): >1400 mg/L 96 h Crystal Violet: EC50, Water Flea (Daphnia magna): < 5 mg/L 48 h Ammonium Oxalate, Monohydrate: No information found. Persistence and Degradability: Some components of this product are not readily biodegradable. Environmental Effects: Hazardous to aquatic organisms. Avoid release to the environment. 13. DISPOSAL INFORMATION Disposal Instructions: All wastes must be handled in accordance with local, state, and federal regulations. Minimize exposure to product waste (see Section 8). Product: Gram’s Stain Kit #1 – Crystal Violet Revision Date: 10/06/2016 6/8
